Personally, I would say no. I consider myself having been a serious gamer for decades, now. USB 3.0 is mostly about bandwidth for data throughput...so it's not likely, even with high-end gaming mice, that you're doing to catch any real benefit over 2.0. You're better bet is to focus on the tech behind mouse-to-surface-scans (such as Darkfield), battery life, and wireless tech (E.g. bluetooth vs RF, etc.).